Table 2. Observation of general symptoms and weight measurement

	Period	Frequency
Observation of general symptoms	Acclimatization period	Once a day
	Pre-administration period	Once a day
	On the day of administration	8 times a day (before administration, immediately after administration, every hour until 6 hours after administration)
	Observation period	Once a day
	Autopsy date	1 time before autopsy
Weight measurement	When receiving animals	1 time
	Acclimatization period	1 time
	Group separation	1 time
	Observation period	5 time (Day 1 [before administration], 2, 6, 9 and 12)
	Autopsy date	1 time before autopsy