Table 3. Mortality and clinical signs in dogs in the CPSS single oral dose escalating toxicity study

Sex	Group	No. of animals	Dose escalation (mg/kg B.W.)	Clinical signs	Mortality	Approximate MTD (mg/kg B.W.)
Male	Vehicle	1	0 → 0 → 0	No abnormalities detected	0% (0/1)1)	> 2,000
	CPSS	2	500	No abnormalities detected	0% (0/2)
			→ 1,000	No abnormalities detected	0% (0/2)
			→ 2,000	No abnormalities detected	0% (0/2)
Female	Vehicle	1	0 → 0 → 0	No abnormalities detected	0% (0/1)1)	> 2,000
	CPSS	2	500	No abnormalities detected	0% (0/2)
			→ 1,000	No abnormalities detected	0% (0/2)
			→ 2,000	No abnormalities detected	0% (0/2)

No. of dead animals/No. of tested animals.
CPSS, collagen peptide derived from skate (R. kenojei) skin; B.W., body weight; MTD, maximum tolerated dose.