Table 1. Primer sequences used in the quantitative real-time PCR

Gene	NCBI reference sequence or gene ID	Primer sequence
mouse HO-1	NM_010442.2	Forward(5’ → 3’): ACA GAT GGC GTC ACT TCGReverse(3’ → 5’): TGA GGA CCC ACT GGA GGA
mouse Nrf2	BC026943.1	Forward(5’ → 3’): ACA TGG AGC AAG TTT GGC AGReverse(3’ → 5’): TGG AGA GGA TGC TGC TGA AA
mouse iNOS	NM_001313922.1	Forward(5’ → 3’): CAT GCT ACT GGA GGT GGG TGReverse(3’ → 5’): CAT TGA TCT CCG TGA CAG CC
mouse COX-2	NM_011198.5	Forward(5’ → 3’): GAA GTC TTT GGT CTG GTG CCT GReverse(3’ → 5’): GTC TGC TGG TTT GGA ATA GTT GC
mouse TNF-α	NM_001278601.1	Forward(5’ → 3’): AGC ACA GAA AGC ATG ATC CGReverse(3’ → 5’): CTG ATG AGA GGG AGG CCA TT
mouse IL-1β	NM_008361.4	Forward(5’ → 3’): TGC AGA GTT CCC CAA CTG GTA CAT CReverse(3’ → 5’): GTG CTG CCT AAT GTC CCC TTG AAT C
mouse IL-6	NM_001314054.1	Forward(5’ → 3’): GAG GAT ACC ACT CCC AAC AGA CCReverse(3’ → 5’): AAG TGC ATC ATC GTT GTT CAT ACA
mouse TLR4	NM_021297.3	Forward(5’ → 3’): AGC TTC TCC AAT TTT TCA GAA CTT CReverse(3’ → 5’): TGA GAG GTG GTG TAA GCC ATG C
mouse NF-κB	NM_008689.3	Forward(5’ → 3’): AGG ATT TGC TGA GGG TTG GGReverse(3’ → 5’): TGG CAT TTA GAC CTT CCC CAT
mouse G-CSF	BC120761.1	Forward(5’ → 3’): CCC TGG AGC AAG TGA GG AAGReverse(3’ → 5’): AGG CGC CCT TGA GTT TGG TG
mouse GM-CSF	X03019.1	Forward(5’ → 3’): GTA GAG GCC ATC AAA GAA GCReverse(3’ → 5’): AGG CGC CCT TGA GTT TGG TG
mouse TGF-β	XM_036152883	Forward(5’ → 3’): TGA TAC GCC TGA GTG GCT GTC TReverse(3’ → 5’): CAC AAG AGC AGT GAG CGC TGA A
mouse IL-10	NM_010548.2	Forward(5’ → 3’): CGG GAA GAC AAT AAC TGC ACC CReverse(3’ → 5’): CGG TTA GCA GTA TGT TGT CCA GC
mouse IFN-γ	NM_008337.4	Forward(5’ → 3’): CTT CTT CAG CAA CAG CAA GGCReverse(3’ → 5’): TCA GCA GCG ACT CCT TTT CC
mouse MCP-1	NM_008570.1	Forward(5’ → 3’): CCC AAT GAG TAG GCT GGA GAReverse(3’ → 5’): TCT GGA CCC ATT CCT TCT TG
mouse GAPDH	NM_001411843	Forward(5’ → 3’): TGC CAC CCA GAA GAC TGT GGReverse(3’ → 5’): TTC AGC TCT GGG ATG ACC TT

PCR, polymerase chain reaction; HO-1, heme oxygenase-1; Nrf2, nuclear factor erythroid 2–related factor 2; iNOS, inducible nitric oxide synthase; COX-2, cyclooxygensase-2; TNF-α, tumor necrosis factor-α; IL, interleukin; TLR4, toll-like receptor 4; NF-κB, nuclear factor kappa B; G-CSF, granulocyte colony-stimulating factor; GM-CSF, granulocyte-macrophage colony-stimulating factor; TGF-β, transforming growth factor-β; IFN-γ, interferon-γ; MCP-1, monocyte chemoattractant protein-1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.