Table 1. Nucleotide sequences of primers used for PCR amplification

Target organisms		Target gene	Sequence (5’→3’)	Product size (bp)	Reference
Bacteria	Brucella abortus	16s RNA	TCGAGCGCCCGCAAGGGG	905	[3]
	Brucella abortus	16s RNA	AACCATAGTGTCTCCACTAA	905	[3]
	Coxiella burnetii	IS1111	TATGTATCCACCGTAGCCAGTC	687	[4]
	Coxiella burnetii	IS1111	CCCAACAACACCTCCTTATTC	687	[4]
	Listeria monocytogenes	hly	CGGAGGTTCCGCAAAAGATG	234	[5]
	Listeria monocytogenes	hly	CCTCCAGAGTGATCGATGTT	234	[5]
	Leptospiraspp.	secY	CTGAATCGCTGTATAAAAGT	285	[6]
	Leptospiraspp.	secY	GGAAAACAAATGGTCGGAAG	285	[6]
	Chlamydophila abortus	pmp	ATGAAACATCCAGTCTACTGG	300	[7]
	Chlamydophila abortus	pmp	TTGTGTAGTAATATTATCAAA	300	[7]
Protozoa	Neospora caninum	Nc-5	CTCGCCAGTCAACCTACGTCTTCT	350	[8]
	Neospora caninum	Nc-5	CCCAGTGCGTCCAATCCTGTAAC	350	[8]
	Toxoplasma gondii	B1	GGAACTGCATCCGTTCATGA	501	[9]
	Toxoplasma gondii	B1	CAGACGAATCACGGAACTG	501	[9]
Virus	IBRV	gC	TACGACTCGTTCGCGCTCTC	476	[10]
Virus	IBRV	gC	GGTACGTCTCCAAGCTGCCC	476	[10]

PCR, polymerase chain reaction; IBRV, infectious bovine rhinotrachitis virus.