Table 3. Animal experimental design for vaccine efficacy evaluation using the developed FAdV-4 challenge model

Group	No1)	Immunization				Challenge
		Age (d)	Virus	Virus titer (TCID50/dose)	Route	Age (d)	Virus	Virus titer (TCID50/dose)	Route
A	9	1	FAdV-4	4.54 × 106	PO	14	YS4	6.31 × 108	IM
B	9	1	FAdV-4	4.54 × 106	PO	14	–	–	–
C	9	1	–	–	–	14	YS4	6.31 × 108	IM
D	9	1	–	–	–	14	–	–	–

Number of chickens.
FAdV-4, fowl adenovirus serotype-4; PO, per os; IM, intramuscular.