Table 2. Analytical conditions of LC-MS/MS

Part NASKA2 (LC)-Triple Quad 4500 (MS/MS)
Column Osaka Soda Capcell pak C18 (4.6 mm × 35 mm, 5 μm)
Injection volume 5 μL
Mobile phase A : 5 mM Ammonium formate in waterB : 5 mM Ammonium formate in MeOH
Oven temp. Time (min) A (%) B (%)
0.00 75.0 15.0
1.00 75.0 15.0
1.50 30.0 60.0
10.00 10.0 90.0
15.00 10.0 90.0
20.00 2.0 98.0
20.10 75.0 15.0
Flow rate 0.25 mL/min
Oven temp. 40°C
Ion source ESI (electrospray ionization)