Table 2. Analytical conditions of LC-MS/MS

Part	NASKA2 (LC)-Triple Quad 4500 (MS/MS)
Column	Osaka Soda Capcell pak C18 (4.6 mm × 35 mm, 5 μm)
Injection volume	5 μL
Mobile phase	A : 5 mM Ammonium formate in waterB : 5 mM Ammonium formate in MeOH
Oven temp.	Time (min)	A (%)	B (%)
	0.00	75.0	15.0
	1.00	75.0	15.0
	1.50	30.0	60.0
	10.00	10.0	90.0
	15.00	10.0	90.0
	20.00	2.0	98.0
	20.10	75.0	15.0
Flow rate	0.25 mL/min
Oven temp.	40°C
Ion source	ESI (electrospray ionization)