Table 1. Nucleotide sequences of primers used for PCR amplification

Target organisms		Target gene	Sequence (5’→ 3’)	Product size (bp)	References
Bacteria	Brucella abortus	16s RNA	TCGAGCGCCCGCAAGGGGAACCATAGTGTCTCCACTAA	905	[20]
	Coxiella burnetii	is1111	TATGTATCCACCGTAGCCAGTCCCCAACAACACCTCCTTATTC	687	[21]
	Listeria monocytogenes	Hly	CGGAGGTTCCGCAAAAGATGCCTCCAGAGTGATCGATGTT	234	[22]
	Leptospira spp.	secY	CTGAATCGCTGTATAAAAGTGGAAAACAAATGGTCGGAAG	285	[23]
	Chlamydophila abortus	Pmp	ATGAAACATCCAGTCTACTGGTTGTGTAGTAATATTATCAAA	300	[24]
Protozoa	Neospora caninum	Nc-5	CTCGCCAGTCAACCTACGTCTTCTCCCAGTGCGTCCAATCCTGTAAC	350	[25]
	Toxoplasma gondii	B1	GGAACTGCATCCGTTCATGACAGACGAATCACGGAACTG	501	[26]
Virus	IBRV	gC	TACGACTCGTTCGCGCTCTCGGTACGTCTCCAAGCTGCCC	476	[27]

PCR, polymerase chain reaction; IBRV, infectious bovine rhinotrachitis virus.