Table 3. Bestkeeper software statistical analysis of housekeeping gene in each tissues of long-tailed chicken

(A) Bestkeeper analysis results in heart tissues
	B2M	SDHA	GAPDH	RPL13	VIM	EEF1A1	HPRT1	GUSB	RPL19	ACTB	ABL1
N	3	3	3	3	3	3	3	3	3	3	3
GM [Ct]	19.47	23.67	19.26	23.19	24.09	19.97	23.33	27.27	23.32	20.16	25.13
AM [Ct]	19.50	23.69	19.27	23.19	24.10	19.98	23.34	27.27	23.34	20.18	25.13
Min [Ct]	18.24	22.92	18.79	22.91	23.50	19.31	22.63	26.90	22.73	19.08	24.52
Max [Ct]	20.94	24.63	19.97	23.46	24.86	20.68	24.50	27.73	24.37	20.97	25.97
SD [± Ct]	0.96	0.63	0.46	0.19	0.51	0.47	0.77	0.31	0.69	0.73	0.56
CV [% Ct]	4.90	2.66	2.41	0.82	2.12	2.36	3.30	1.12	2.95	3.63	2.22
Max [x-fold]	–2.35	–1.68	–1.39	–1.22	–1.51	–1.58	–1.63	–1.29	–1.51	–2.12	–1.52
Max [x-fold]	2.76	1.94	1.63	1.20	1.71	1.64	2.25	1.38	2.06	1.75	1.79
SD [± x-fold]	1.94	1.55	1.38	1.14	1.42	1.39	1.71	1.24	1.61	1.66	1.47

(B) Bestkeeper analysis results in thigh tissues
	B2M	SDHA	GAPDH	RPL13	VIM	EEF1A1	HPRT1	GUSB	RPL19	ACTB	ABL1
N	3	3	3	3	3	3	3	3	3	3	3
GM [Ct]	19.87	25.13	16.47	27.02	23.23	20.06	24.02	26.78	21.80	19.75	27.36
AM [Ct]	19.88	25.14	16.47	27.02	23.23	20.08	24.02	26.80	21.81	19.77	27.37
Min [Ct]	18.98	24.63	15.98	26.57	22.95	18.91	23.83	26.18	20.92	18.95	26.55
Max [Ct]	20.79	25.87	16.73	27.54	23.79	20.94	24.16	27.90	22.69	20.71	27.90
SD [± Ct]	0.61	0.49	0.33	0.35	0.37	0.78	0.13	0.73	0.59	0.63	0.55
CV [% Ct]	3.05	1.94	2.00	1.28	1.60	3.89	0.52	2.74	2.72	3.18	2.00
Max [x-fold]	–1.85	–1.42	–1.41	–1.36	–1.22	–2.22	–1.14	–1.52	–1.84	–1.75	–1.76
Max [x-fold]	1.90	1.66	1.20	1.43	1.48	1.84	1.10	2.16	1.86	1.94	1.45
SD [± x-fold]	1.52	1.40	1.26	1.27	1.29	1.72	1.09	1.66	1.51	1.55	1.46

(C) Bestkeeper analysis results in breast tissues
	B2M	SDHA	GAPDH	RPL13	VIM	EEF1A1	HPRT1	GUSB	RPL19	ACTB	ABL1
N	3	3	3	3	3	3	3	3	3	3	3
GM [Ct]	20.43	24.28	15.25	25.30	23.14	20.39	23.35	26.07	22.13	19.43	27.07
AM [Ct]	20.44	24.29	15.26	25.31	23.15	20.40	23.36	26.08	22.13	19.44	27.07
Min [Ct]	19.57	23.78	14.95	24.93	22.20	19.61	22.90	25.42	21.92	18.97	26.60
Max [Ct]	20.94	24.92	15.47	26.06	23.92	20.96	23.92	26.92	22.52	20.38	27.71
SD [± Ct]	0.58	0.42	0.20	0.50	0.63	0.52	0.38	0.56	0.26	0.63	0.42
CV [% Ct]	2.83	1.74	1.34	1.98	2.73	2.56	1.61	2.15	1.17	3.22	1.56
Max [x-fold]	–1.81	–1.42	–1.24	–1.29	–1.92	–1.71	–1.37	–1.57	–1.16	–1.38	–1.39
Max [x-fold]	1.42	1.55	1.16	1.69	1.71	1.48	1.48	1.80	1.31	1.93	1.56
SD [± x-fold]	1.49	1.34	1.15	1.41	1.55	1.44	1.30	1.48	1.20	1.54	1.34

N, number of samples; Ct, threshold cycle value; GM, geometric mean; AM, arithmetic mean; Min, minimal value; Max, maximal value; SD, standard deviation; CV, coefficient of variance.