Table 2. Melt temperatures and efficiency values of housekeeping genes

Gene	Annealing temperature (°C)	Melt temperature (mean ± SD, °C)	Efficiency (%)
B2M	60	85.5 ± 0.2	100
SDHA	60	79.1 ± 0.2	106
GAPDH	60	83.8 ± 0.2	91
RPL13	60	81.2 ± 0.2	94
VIM	60	79.4 ± 0.2	95
EEF1A1	60	81.9 ± 0.2	95
HPRT1	60	78.6 ± 0.2	104
GUSB	60	81.6 ± 0.2	101
RPL19	60	82.7 ± 0.2	100
ACTB	60	80.9 ± 0.1	94
ABL1	60	83.7 ± 0.1	81