Table 1. Primers used for subcloning of expression constructs

Constructs Primer sequences
pET21a-LOX F: 5’-CTAGGGGCTAGCGACGACCCTTACAACCCCTA-3’NheIR: 5’-TTACCCAAGCTTATACGGTGAAATTGTGCAGCCTG-3’HindIII
pET21a-LOX-v2 F: 5’-GCGGCTAGCATGTCCATGTACAACCTGAG-3’NheIR: 5’-GCGAAGCTTATACGGTGAAATTGTGCAGC-3’HindIII
pFLAG-LOX F: 5’-CCAGAATTCCACCATGCGCTTCGCCTGGACCG-3’EcoR IR: 5’-CGCGGATCCATACGGTGAAATTGTGCAGCC-3’BamH I
pFLAG-LOX-v2 F: 5’CCAGAATTCCACCATGTCCATGTACAACCTGAG-3’EcoR IR: 5’-CACGGTACCATACGGTGAAATTGTGCAGC-3’Kpn I
The underlined bold letters indicate the unique restriction sites used for subcloning.
LOX, lysyl oxidase; LOX-v2, lysyl oxidase-variant 2.