Table 1
Mycoplasma species PCR primers and reaction conditions

Species	Primer sequence	Cycle conditions (×30)	Product size (bp)
M. canis	For 5′-CACCGCCCGTCACACCA-3′	95°C 1 min,	247
	Rev 5′-CTGTCGGGGTTATCTCGAC-3′	55°C 30 s, 72°C 1 min
M. felis	For 5′-CACCGCCCGTCACACCA-3′	95°C 45 s, 51°C 30 s,	238
	Rev 5′-GGACTATTATCAAAAGCACATAAC-3′	72°C 20 s