Table 2
Analysis of sperm motility in antibiotic-free BTS in the presence of RLSE1

Parameters		Antibiotics in BTS	RLSE (μg/mL) in ntibiotic-free BTS
			0	0.1	0.5	1	2	5	10	DMSO
			Total motile spermatozoa (%)		87.0 ± 0.8a	59.5 ± 4.6bc	60.8 ± 8.9bcd	70.0 ± 3.9b	67.1 ± 2.7b	59.3 ± 3.7bcd	59.0 ± 2.8bcd	43.8 ± 7.0cd	42.3 ± 9.1d
Progressive motile spermatozoa (%)		70.8 ± 5.2a	23.8 ± 2.0bc	25.5 ± 5.0bc	31.5 ± 3.4b	32.4 ± 8.5b	25.6 ± 2.5bc	20.0 ± 6.0bc	9.0 ± 2.0c	33.7 ± 14.9b
Hyperactive spermatozoa (%)		0.2 ± 0.2	0.4 ± 0.4	1.6 ± 0.8	1.8 ± 0.3	2.0 ± 0.8	2.1 ± 0.8	1.1 ± 0.6	0.6 ± 0.3	1.2 ± 0.7
Kinetic values2	VCL (µm/s)	69.4 ± 5.6a	42.1 ± 1.2bc	43.5 ± 3.8b	47.9 ± 3.3b	50.5 ± 8.9b	46.4 ± 1.0b	37.6 ± 7.9bc	26.9 ± 2.6c	39.1 ± 1.7bc
	VSL (µm/s)	20.3 ± 2.3a	9.8 ± 0.9bc	10.0 ± 0.9bc	10.6 ± 0.2b	11.4 ± 3.0b	10.6 ± 1.0b	8.5 ± 2.1bc	5.3 ± 0.8c	7.9 ± 0.4bc
	VAP (µm/s)	34.7 ± 3.4a	19.4 ± 1.1b	19.9 ± 1.8b	21.5 ± 1.1b	23.1 ± 5.1b	21.1 ± 1.0b	17.0 ± 4.3bc	10.6 ± 1.5c	16.9 ± 0.8bc
	LIN (%)	27.8 ± 2.1a	20.5 ± 3.4abc	22.1 ± 1.2b	20.9 ± 4.4abc	17.7 ± 3.3bc	17.4 ± 1.8bc	18.0 ± 2.0bc	12.3 ± 1.9c	16.6 ± 2.3bc
	STR (%)	54.5 ± 2.7a	45.5 ± 4.3b	47.6 ± 0.9b	44.2 ± 4.3b	42.1 ± 4.2b	44.7 ± 2.5b	45.0 ± 1.2b	41.8 ± 4.0b	41.5 ± 3.5b
	WOB (%)	48.2 ± 1.7a	40.6 ± 2.9b	41.8 ± 0.8b	41.8 ± 3.5b	39.0 ± 2.8b	36.8 ± 1.9bc	37.6 ± 2.6b	29.4 ± 3.3bc	36.6 ± 2.2bc

Experiments were repeated three times with three different boars. Values are expressed as mean SEM.
VCL: curvilinear velocity, VSL: straight-line velocity, VAP: average path velocity, LIN: linearity, STR: straightness, and WOB: wobble.
Means in the same row with different superscripts differ significantly (p<0.05).