Table 5.
Changes in numbers of inflammatory cells, fibroblasts (fibrocytes), and micro-vessels in granulation tissues at 20 days after application with re-epithelialization
Histomorphometry | Control | Madecassol™ 100 mg/kg | β-glucan-applied groups |
10 mg/kg | 50 mg/kg | 100 mg/kg |
Cell counts (N/mm2) | | | | | |
PMNs1) | 98.60 ± 26.72 | 58.90 ± 14.40* | 40.60 ± 12.52* | 24.00 ± 8.82* | 16.10 ± 6.82* |
Macrophages | 33.30 ± 9.21 | 23.80 ± 8.95** | 20.90 ± 5.32* | 10.30 ± 3.06* | 8.00 ± 2.31* |
Lymphocytes | 40.30 ± 9.25 | 28.10 ± 10.91** | 26.80 ± 12.53** | 20.60 ± 4.62* | 17.80 ± 8.13* |
Fibroblasts | 32.50 ± 10.86 | 76.00 ± 23.84* | 63.10 ± 14.18* | 102.80 ± 20.81* | 125.20 ± 35.70* |
|
Angiogenesis (N/mm2) | | | | | |
Micro-vessels | 67.30 ± 21.31 | 68.70 ± 26.30 | 90.50 ± 25.29 | 107.00 ± 25.63* | 109.20 ± 17.30* |
Re-epithelialization (%) | 27.18 ± 10.64 | 46.67 ± 10.56* | 30.22 ± 8.42 | 69.77 ± 12.39* | 80.18 ± 11.49* |
n=10; (Mean ± S.D.);
Polymorphonuclear leukocytes; *p<0.01 and **p<0.05 compared to that of control by MW test.