Table 2.
Information on primers and restriction enzymes for PCR-RFLP

Primer Name	Sequence (5' to 3')	Tm (°C)	Product Size (bp)	Restriction Enzyme	Ref.
GBP1_F	GGATAACACTTCGGTAACTTGC	58	587	Bsu36I	[11]
GBP1_R	GAAGGGGAAACTGAGACACAAT	58	587	Bsu36I	[11]
GBP2_F	ACGGGAACTCCGAAGCAACT	60	325	Ssp I	[11]
GBP2_R	CAAGGGCTTTCCCACTGTCC	60	325	Ssp I	[11]
CD163_F	GACCTGGACTATTGAATGGC	53	139	Fnu4h I	[21]
CD163_R	CATACAACTTTGAGTAGTCACTTC	53	139	Fnu4h I	[21]
CD169_F	CGGCTGCTGTAGCTCTGATTG	61	530	Hinp1 I	[21]
CD169_R	GCCTTGGCCTGGTTTCCTTA	61	530	Hinp1 I	[21]

Where, “F” and “R” in primer names represent “Forward” and “Reverse”.
Where c.=coding DNA reference sequence; p.=protein reference sequence; [] indicates an allele;
(asterisk)=translation termination (stop) codon. The above polymorphisms were named according to the nomenclature for the description of sequence polymorphisms (http://www.hgvs.org/mutnomen/).