Table 2.
PCR primers and restriction enzymes used for genotyping four SNPs

Gene & SNP	Reference ID	Primer sequences (5’→3’)	Fragment size (bp)	TA (°C)	Restriction Enzyme (°C)	Size (bp) of the allelic polymorphism
DSP	rs45430738	CAATGGCGTGACCGATGG	472 bp	60°C	Hpy188I	307, 263, 165 bp
G>A		TGTGGCTGTGGCGTAGGCT			(37°C)
RASA2	rs45431691	GTGCTAAAACTGCCTAAG	102 bp	54°C	MseI	102, 60 bp
A>C		AACCCTAGCACTGTATGG			(37°C)
MYH3	rs45431421	CGCCGAAAGCTCACTGTG	265 bp	55°C	BslI	265, 214 bp
C>A		CTGGGGAGGAACCTGCTG			(55°C)
GSN	rs45435111	CGGGAACCTGCAGTATGA	291 bp	56°C	EcoRV	291, 245 bp
G>A		GGTGGTCTGGTGCGATCA			(37°C)